Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:31
Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1159111_sequence (Version 1) |
Description
This part encodes a membrane-associated Thermonuclease NucA for the chassis Physcomitrella patens. NucA is a endo/exonuclease that degrades ssDNA/dsDNA/ssRNA/dsRNA. It cannot execute its deadly potential when it is bound to the cytoplasmatic membrane. The region between NucA and the transmembrane domain consists a long linker with a TEV cleavage site and a SV40 nuclear localization signal (NLS). By cleaving the TEV cleavage site with a TEV Protease the nuclease NucA translocates in the nucleus due to its SV40 NLS and degrades the genomic DNA resulting a inevitable induction of programmed cell-death (PCD). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.Notes
xSource
Synthetic| Access | Instance | Definition |
| public public public | BBa_K1159314 BBa_K1159993 BBa_K1159110 | BBa_K1159314 BBa_K1159993 BBa_K1159110 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1159314 BBa_K1159993 BBa_K1159110 | 1,292 293,298 299,916 | BBa_K1159314 BBa_K1159993 BBa_K1159110 |