Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Hall
Date created: 2008-10-06 11:00:00
Date modified: 2015-05-08 01:09:37
dxs+LIMS1+appY
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K118024_sequence (Version 1) |
Description
dxs, from Escherichia coli JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].lims1, from Citrus limon, encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway). (+)-Limonene is a component of lemon scent.
appY, from Escherichia coli JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
(All three genes are individually preceded by a ribosome binding site.)
Notes
The order of the genes in the operon corresponds to our perceived importance of each gene, bearing in mind that translation is more efficient of the genes early in the mRNA.Source
dxs and appY are both from E. coli JM109 genomic DNA.lims1 is from Citrus limon genomic DNA.
| Access | Instance | Definition |
| public public public public public public | BBa_J15001 BBa_K118000 BBa_J15001 BBa_I742110 BBa_J15001 BBa_K118001 | BBa_J15001 dxs BBa_J15001 LIMS1 BBa_J15001 appY |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J15001 BBa_K118000 BBa_J15001 BBa_I742110 BBa_J15001 BBa_K118001 | 1,10 17,1882 1891,1900 1907,3577 3586,3595 3602,4354 | BBa_J15001 dxs BBa_J15001 LIMS1 BBa_J15001 appY |