Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chris French
Date created: 2008-10-22 11:00:00
Date modified: 2015-05-08 01:09:37
limonene generator with lac promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K118025_sequence (Version 1) |
Description
This part consists of a lac promoter (with lacZ' tag for blue-white selection) coupled to the limonene generation system consisting of dxs (deoxyxylulose phosphate synthase, rate controlling step of terpenoid biosynthesis in bacteria) + LIMS1 (limonene synthase 1 from lemon) + appY (a transcription factor known to increase terpenoid biosynthesis).Notes
No special considerations.Source
The lac promoter, dxs and appY are from E. coli; LIMS1 is from lemon.| Access | Instance | Definition |
| public public public public public public public | BBa_J33207 BBa_J15001 BBa_K118000 BBa_J15001 BBa_I742110 BBa_J15001 BBa_K118001 | BBa_J33207 BBa_J15001 dxs BBa_J15001 LIMS1 BBa_J15001 appY |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_J15001 BBa_K118000 BBa_J15001 BBa_I742110 BBa_J15001 BBa_K118001 | 1,600 609,618 625,2490 2499,2508 2515,4185 4194,4203 4210,4962 | BBa_J33207 BBa_J15001 dxs BBa_J15001 LIMS1 BBa_J15001 appY |