Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Amy Soon
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41
pBAD+Riboswitch2+GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1212009_sequence (Version 1) |
Description
Testing construct. GFP expression is controlled by arabinose and theophylline via the pBAD promoter and the theophylline riboswitch.Notes
The end of the riboswitch sequence must be directly before the start codon of the coding sequence for GFP. There cannot be a scar.Source
pBAD and GFP are from the iGEM distribution kit plates and the riboswitch is from the Gallivan Lab's paper A flow cytometry-based screen for synthetic riboswitches.by Lynch et al. in Appl Environ Microbiol.
| Access | Instance | Definition |
| public public public | BBa_K206000 BBa_K1212000 BBa_K1212019 | pBAD BBa_K1212000 BBa_K1212019 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K206000 BBa_K1212000 BBa_K1212019 | 1,130 131,186 187,1086 | pBAD BBa_K1212000 BBa_K1212019 |