Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margaret Ruzicka
Date created: 2008-10-25 11:00:00
Date modified: 2015-07-09 01:36:31
Broad-Host-Range RSF1010 derived plasmid
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K125010_sequence (Version 1) |
Description
This Broad-Host-Range plasmid is derived from RSF1010. The replication proteins, the origin of vegetative replication, and the aadA gene were inserted into the [[Part:BBa_I51020|Base Vector]]. The MPF system has been removed and replaced with the RP4 origin of transfer.This plasmid has been converted to BioBrick format and is also more compact that the original version. The replication proteins with the origin of vegetative replication allows for autonomous replication in a broad range of gram negative bacteria. The oriT allows for mobilization by the self-transmissible plasmid RP4. The aadA region allows resistance to Spectinomycin and Streptomycin. The Base Vector provides an insulated BioBrick cloning site containing a high copy origin and the ccdB [[Featured Parts:Cell Death|death gene]] which are both removed when a part in cloned in. This allows one to make a lot of the plasmid for cloning, and a means for positive selection.
Notes
PCR amplification was used to isolate the parts from pRL1383a. The oriT,lac promoter, and rbs were all synthesized.Source
This part is derived from pRL1383a, an RSF1010 derived plasmid and the BioBrick [[Part:BBa_I51020|Base Vector]].| Access | Instance | Definition |
| public public public public public public public public | BBa_I14032 BBa_B0034 BBa_K125800 BBa_I14032 BBa_B0034 BBa_K125530 BBa_K125320 BBa_I51020 | P(Lac) IQ BBa_B0034 Rep P(Lac) IQ BBa_B0034 BBa_K125530 OriT-RP4 BB base ve |