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pUV + NhaS

BBa_K1255002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1255002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Fernanda Puente, Marina Emilio

Date created: 2014-06-15 11:00:00

Date modified: 2015-05-08 01:09:45

NhaS with an RFP reporter induced by an UV promoter.

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1255002_sequence (Version 1)

Description

NhaS with an RFP reporter induced by an UV promoter.

This device is activated when UV promoter (BBa_I765001) is exposed to 360nm of UV irradiation. It produces a gene called NhaS (BBa_K1255000) that is a sodium ion binding protein and reports its function with a red fluorescence reporter. (BBa_E1010)
It contains an RBS (BBa_B0034) before its genes.

Notes

NhaS (BBa_K1255000) sequence was optimized for its expression in E. coli

Source

RFP comes from Discosoma striata (coral) and NhaS from Bacillus firms

Components

Access	Instance	Definition
public public public public public	BBa_I765001 BBa_B0034 BBa_K1255000 BBa_B0034 BBa_E1010	pUV BBa_B0034 nhaS BBa_B0034 mRFP1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I765001 BBa_B0034 BBa_K1255000 BBa_B0034 BBa_E1010	1,76 85,96 103,309 318,329 336,1041	pUV BBa_B0034 nhaS BBa_B0034 mRFP1

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K1255002/1