Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-12 11:00:00
Date modified: 2015-05-08 01:09:50
yqjF promoter coupled to mKate2 reporter gene and ybiJ promoter coupled to mKate2 reporter gene
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K1316009_sequence (Version 1) |
Description
Promoter of the yqjF and ybiJ genes, which are activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.
nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoter
This construct is, then, designed to be able to detect and quantify the presence of compunds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB. The presence of the N-genes aims to enhance this response.
Having both promoters in the same plasmid aims to improve the cellular response in front of the inducing chemical compounds (2,4,6-TNT, 2,4-DNT and 1,3-DNB).
Notes
A double terminator was placed between both yqjF+mKate2 and ybiJ+mKate2 to avoid having interfering unfinished transcripts of the 2nd mKate2 copySource
Promoters from Belkin laboratory (Belkin et al. Appl Microbiol Biotechnol (2014) 98:885???895)| Access | Instance | Definition |
| public public | BBa_K1316003 BBa_K1316005 | BBa_K1316003 BBa_K1316005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1316003 BBa_K1316005 | 1,1099 1108,2359 | BBa_K1316003 BBa_K1316005 |