folKE + b0

BBa_K137054 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137054
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:10:09

Constitutive folKE expression construct



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K137054_sequence (Version 1)

Description

This is the folate synthesis gene folKE, one of five genes (folB, folKE, folP, folC, folA) involved in the folate synthesis operon. In this construct, folKE has been inserted behind the strong promoter j23100, the strong RBS b0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid psB2K3, which can be induced to high copy via IPTG.

The purpose of this construct is to test the effects of folKE (a fusion gene for GTP cyclohydrolase I (folE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (folK)) overexpression on total folate production in Escherichia coli, with the goal of increasing total folate production.

Notes

FolKE has been shown to be a good target for metabolically engineering the overexpression of folate in bacteria, particularly in L.lactis. One possible explanation for this is that since GTP cyclohydrolase I is involved in the first enzymatic step in the folate biosynthesis pathway, overexpression of this enzyme will increase the total flux through the pathway.

Source

The original folKE gene was extracted from the L.lactis subspe. IL1403 genome.

igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K137054/1