Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:10:09
Constitutive folKE expression construct
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K137054_sequence (Version 1) |
Description
This is the folate synthesis gene folKE, one of five genes (folB, folKE, folP, folC, folA) involved in the folate synthesis operon. In this construct, folKE has been inserted behind the strong promoter j23100, the strong RBS b0034, and in front of the double terminator B0015. The vector is the inducible copy plasmid psB2K3, which can be induced to high copy via IPTG.The purpose of this construct is to test the effects of folKE (a fusion gene for GTP cyclohydrolase I (folE) + 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (folK)) overexpression on total folate production in Escherichia coli, with the goal of increasing total folate production.
Notes
FolKE has been shown to be a good target for metabolically engineering the overexpression of folate in bacteria, particularly in L.lactis. One possible explanation for this is that since GTP cyclohydrolase I is involved in the first enzymatic step in the folate biosynthesis pathway, overexpression of this enzyme will increase the total flux through the pathway.Source
The original folKE gene was extracted from the L.lactis subspe. IL1403 genome.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K137011 BBa_B0010 BBa_B0012 | BBa_J23100 BBa_B0034 folKE BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K137011 BBa_B0010 BBa_B0012 | 1,35 44,55 62,1114 1123,1202 1211,1251 | BBa_J23100 BBa_B0034 folKE BBa_B0010 BBa_B0012 |