BBa_K137057

BBa_K137057 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K137057

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Allen Lin

Date created: 2008-07-30 11:00:00

Date modified: 2015-05-08 01:10:09

GFP fimE switch with 150 bp tetR promoter

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K137057_sequence (Version 1)

Description

A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.

Notes

None.

Source

Previously constructed Biobrick parts.

Components

Access	Instance	Definition
public	BBa_K137010	fimE IRL
public	BBa_K137046	BBa_K137046
public	BBa_K137008	IRR
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K137010	1,35	fimE IRL
BBa_K137046	44,193	BBa_K137046
BBa_K137008	202,236	IRR
BBa_B0034	245,256	BBa_B0034
BBa_E0040	263,982	GFP
BBa_B0010	991,1070	BBa_B0010
BBa_B0012	1079,1119	BBa_B0012

Other Properties

• igem#experience: Fails
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137057/1

Member of these Collections

• //function/recombination/fim
• iGEM Parts Registry