BBa_K1398001

BBa_K1398001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1398001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Exeter iGEM 2014

Date created: 2014-08-10 11:00:00

Date modified: 2015-05-08 01:10:15

XenB (Inducible Construct)

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1398001_sequence (Version 1)

Description

A construct created to degraded TNT and nitroglycerine.
The construct contains the coding sequence for XenB (BBa_K1398000), an enzyme with the capability to degrade nitro- groups in chemicals.
The construct also contains an inducible promoter (BBa_R0010), a strong RBS (BBa_B0034) and a double terminator (BBa_B0010).
The protein has been codon-optimised for expression in E. coli.

Notes

This construct was created to allow for the over-expression of XenB, so it contains high expression regulatory sequences. A lactose inducible promoter has been used to give control over expression.

Source

The original source of XenB was Pseudomonas putida. The regulatory sequences were acquired from the iGEM repository.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0034	BBa_B0034
public	BBa_K1398000	XenB
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0034	209,220	BBa_B0034
BBa_K1398000	227,1291	XenB
BBa_B0015	1300,1428	BBa_B0015

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1398001/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //function/degradation
• iGEM Parts Registry