BBa_K1401616

BBa_K1401616 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1401616

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yash Agarwal

Date created: 2014-10-10 11:00:00

Date modified: 2015-05-08 01:10:16

pLac - BCD2 - tetR- GFP

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1401616_sequence (Version 1)

Description

The parts contain the BioBricks - R0010_EB, BCD2_BC, C0040_CI, E0040m_ID and B0015_DF. All of these are MoClo variants i.e. they have MoClo flanking regions attached to each basic part. A new fusion site (I) was specifically designed to create the fusion protein (regulator + reporter).

Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTAGA. These were then put together using the digestion-ligation MoClo reaction. E0040m has fixed illegal cut sites

Source

E. Coli

Components

Access	Instance	Definition
public	BBa_K783046	BBa_K783046
public	BBa_K1114107	BBa_K1114107
public	BBa_K1401600	BBa_K1401600
public	BBa_K1401602	BBa_K1401602
public	BBa_K783020	BBa_K783020

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K783046	1,200	BBa_K783046
BBa_K1114107	209,300	BBa_K1114107
BBa_K1401600	309,1001	BBa_K1401600
BBa_K1401602	1010,1739	BBa_K1401602
BBa_K783020	1748,1876	BBa_K783020

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1401616/1

Member of these Collections

• iGEM Parts Registry