BBa_K1421001

BBa_K1421001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1421001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mengni Wang

Date created: 2014-10-03 11:00:00

Date modified: 2015-05-08 01:10:22

plux R+RBS+LacIM1+2TE

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1421001_sequence (Version 1)

Description

This device is comprised of plux R (BBa_R0062), RBS (BBa_B0034), lacIM1 (BBa_K082005) and 2 TE (BBa_B0010 and BBa_B0012).
The lux pR is a right promoter in the lux cassette of V. Fischeri, which gives weak constitutive expression while enhanced when binding to the complex of LuxR and AHL (acyl-homoserine lactone ).
The LacIM1 is a LacI mutant, which can repress lac promoter. Thanks to the work of Tokyo_Tech 2010, it is confirmed that this mutant protein has a weaker function of repression to the lac promoter, compared to the wild type LacI.

Notes

Try to use a simpler method to combine these several parts together.

Source

From part pool.

Components

Access	Instance	Definition
public	BBa_R0062	lux pR
public	BBa_B0034	BBa_B0034
public	BBa_K082005	BBa_K082005
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0062	1,55	lux pR
BBa_B0034	64,75	BBa_B0034
BBa_K082005	82,1164	BBa_K082005
BBa_B0010	1173,1252	BBa_B0010
BBa_B0012	1261,1301	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1421001/1

Member of these Collections

• iGEM Parts Registry