Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Casey Martin
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:23
Lactose-inducible killswitch, produces SAMS and NAS1
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1422505_sequence (Version 1) |
Description
This plasmid includes a killswitch and produces SAMS (BBa_K1422500) and NAS1 (BBa_K1422501), two of the four enzymes used in the production of corn phytosiderophores. The killswitch (BBa_K1422504) is lactose and IPTG inducible and uses the antiholin Hyb5 to lyse the cell. The corn genes are codon optimized for Bacillus subtilis, and the RBS ("SpoVG" BBa_K143021) has shown high strength in Bacillus subtilis. mRFP is used as a reporter because it is easily observed, easily measured, and expressed in Bacillus subtilis.Notes
The kill switch is located at the beginning of a plasmid to avoid being accidentally translated by an mRNA due to a previous section not being properly terminated. The reporter is placed after the corn genes because gene expression decreases linearly as the distance from the promoter increases, and expressing the corn genes is much more important than expressing the reporter. The corn genes are codon optimized for Bacillus subtilis (details are on their respective Registry pages).Source
Many of the genes (Hyb5, SAMS, and NAS1) come fromNCBI.(2 December, 2008). Lactobacillus phage YB5 holin gene, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/EF623894?report=GenBank NCBI.
NCBI. (2014). S-adenosylmethionine synthetase 1 [Zea mays]. http://www.ncbi.nlm.nih.gov/protein/NP_001148708.1
NCBI. (5 February, 2003). Zea mays ZmNAS1 mRNA, complete cds. http://www.ncbi.nlm.nih.gov/nuccore/19911063
| Access | Instance | Definition |
| public public public public public public public public public | BBa_K1422504 BBa_K143012 BBa_K143021 BBa_K1422500 BBa_K143021 BBa_K1422501 BBa_K143021 BBa_E1010 BBa_B1006 | BBa_K1422504 Pveg RBS-spoVG BBa_K1422500 RBS-spoVG BBa_K1422501 RBS-spoVG mRFP1 BBa_B1006 |