Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Corbyn Lamy
Date created: 2014-09-12 11:00:00
Date modified: 2015-05-08 01:10:23
CAEV capsid protein anchored in cell membrane
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1423004_sequence (Version 1) |
Description
This part is a constitutive promoter, a ribosome binding site, a cell surface anchoring motif, a caprine arthritis encephalitis virus capsid protein domain, and a double terminator. We designed this part to use in our direct agglutination assay. The BCLA NTD will allow the capsid protein domain to be expressed on the outer surface of the cell. The capsid protein domain is an antigen which we then used with antibodies for agglutination.Notes
The CAEV capsid protein needed to be in frame next to the BCLA domain.Source
bacillus anthracis and caprine arthritis encephalitis virus| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1423001 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1423001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1423001 BBa_B0015 | 1,35 44,55 64,126 135,737 746,874 | BBa_J23100 BBa_B0034 BBa_K1423000 BBa_K1423001 BBa_B0015 |