Ph-s-gsiB

BBa_K143054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-07 11:00:00

Date modified: 2015-05-08 01:10:24

Promoter hyper-spank and RBS gsiB for B. subtilis

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K143054_sequence (Version 1)

Description

Inducible promoter hyper-spank(BBa_K143015) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.

Phyperspank-gsiB can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.

IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143035). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.

To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.

Notes

The sequence of promoter hyperspank was obtained from the B. subtilis integration vector pDR111 and RBS-gsiB were obtained from papers1 and the sequence synthesised by GeneArt

Source

Phyperspank-gsiB was synthesised by GeneArt

Components

Access	Instance	Definition
public	BBa_K143015	Ph-s
public	BBa_K143020	RBS-GsiB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143015	1,101	Ph-s
BBa_K143020	110,120	RBS-GsiB

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143054/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry