XylR-T

BBa_K143063 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143063

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-08 11:00:00

Date modified: 2015-05-08 01:10:24

Xylose operon repressor protein - Terminator

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K143063_sequence (Version 1)

Description

Xylose operon repressor protein(XylR)(BBa_K143036) coupled to the double terminator (BBa_B0015.

XylR can be used in conjunction with the xylose operon promoter (BBa_K143014), where the XylR will act as a receiver for a xylose input to result in a Polymerases per second (PoPS) output.

The double terminator is the most commonly used terminator and is a combination of parts BBa_B0010 and BBa_B0012.

The double terminator allows the XylR to be easily incorporated into a closed transcriptional unit.

Notes

XylR was identified from the B. subtilis chromosome. The double terminator is the most commonly used registry terminator.

Source

XylR was produced by PCR cloning using Pfu form the B. subtilis chromosome and cloned into a BioBrick with the registry double terminator

Components

Access	Instance	Definition
public	BBa_K143036	XylR
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143036	1,1056	XylR
BBa_B0010	1065,1144	BBa_B0010
BBa_B0012	1153,1193	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143063/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry