BBa_K143067

BBa_K143067 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K143067

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chris Hirst

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:10:24

AmyE integratable PoPS generator (P43-gsiB)

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K143067_sequence (Version 1)

Description

AmyE 5' Integration sequence(BBa_K143001) coupled to the PoPS generator P43-gsiB (BBa_K143050).

The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(BBa_K143002) is cloned onto the 3' end of the construct.

The P43-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.

Notes

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector pDR111 utilising Pfu DNA polymerase. The sequence of P43-gsiB was obtained from papers.

Source

The amyE 5' integration sequence was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the P43-gsiB that was synthesised by GeneArt.

Components

Access	Instance	Definition
public	BBa_K143001	amyE 5 IS
public	BBa_K143013	P43
public	BBa_K143020	RBS-GsiB

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K143001	1,522	amyE 5 IS
BBa_K143013	531,586	P43
BBa_K143020	595,605	RBS-GsiB

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K143067/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• iGEM Parts Registry