Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Demeulemeester
Date created: 2008-08-19 11:00:00
Date modified: 2015-05-08 01:10:29
FILTER Key (TetR promoter + key)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K145215_sequence (Version 1) |
Description
FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein.The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
Notes
We did a lot of modeling on this device.The most efficient Berkeley 2006 key and lock have been used.
The RiboKy might be required in higher copy number although modeling doesn't predict this.
Source
other parts| Access | Instance | Definition |
| public public public public public | BBa_R0040 BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012 | p(tetR) BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012 | 1,54 63,156 165,261 270,349 358,398 | p(tetR) BBa_J23008 BBa_J23009 BBa_B0010 BBa_B0012 |