Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:39
Delta 9,12 and 15 Desaturase
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1472612_sequence (Version 1) |
Description
This translational unit is composed of delta9, delta 12 and delta 15 desaturase genes with RBS for each of them. Desaturase introduces a cis-carbon double bond to fatty acid carbon chain. ∆ 9 desaturase introduce double bond to stearic acid at the carbon 9 position to produce oleic acid. ∆12 desaturase introduce double bond at carbon 12 of oleic acid so to produce linolenic acid. At the end, ∆15 desaturase convert linolenic acid into alpha-linolenic acid by adding a double bond at carbon 15.Notes
The desaturase genes in plasmids were introduced into E. coli for amplification. The final construct includes iGEM provided RBS (BBa_B0034).Source
All the desaturase sequences are derived from the Synechocystis sp. but optimised for the expression in E. coli.| Access | Instance | Definition |
| public public public public public public | BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 | 1,12 19,978 987,998 1005,2066 2075,2086 2095,3218 | BBa_B0034 BBa_K1027001 BBa_B0034 BBa_K1027002 BBa_B0034 BBa_K1472610 |