BBa_K1532004

BBa_K1532004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1532004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2014-09-29 11:00:00

Date modified: 2015-05-08 01:10:52

Plac->AraC

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1532004_sequence (Version 1)

Description

This part is a functional device with the input of LacI & IPTG and the out put of AraC. The expression of the output signal will be activated if the protein LacI and the small molecule IPTG exists the same time. This device will not work with the existence of glucose.We also added a Kpn I cutting site at each side of this part.

Notes

The bio-brick BBa_R0010 contains a cap-site so the the glucose will affect the expression of this device .So that a glucose-free culture should be used when using this part.

Source

This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.

Components

Access	Instance	Definition
public	BBa_K1532002	BBa_K1532002
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_C0080	araC
public	BBa_B0015	BBa_B0015
public	BBa_K1532002	BBa_K1532002

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1532002	1,10	BBa_K1532002
BBa_R0010	19,218	LacI
BBa_B0030	227,241	BBa_B0030
BBa_C0080	248,1187	araC
BBa_B0015	1196,1324	BBa_B0015
BBa_K1532002	1333,1342	BBa_K1532002

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1532004/1

Member of these Collections

• iGEM Parts Registry