Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyuan Qiu
Date created: 2014-09-29 11:00:00
Date modified: 2015-05-08 01:10:52
Plac->AraC
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1532004_sequence (Version 1) |
Description
This part is a functional device with the input of LacI & IPTG and the out put of AraC. The expression of the output signal will be activated if the protein LacI and the small molecule IPTG exists the same time. This device will not work with the existence of glucose.We also added a Kpn I cutting site at each side of this part.Notes
The bio-brick BBa_R0010 contains a cap-site so the the glucose will affect the expression of this device .So that a glucose-free culture should be used when using this part.Source
This part is assembled with standard bio-bricks from the distribution with the standard assembly protocol.| Access | Instance | Definition |
| public public public public public public | BBa_K1532002 BBa_R0010 BBa_B0030 BBa_C0080 BBa_B0015 BBa_K1532002 | BBa_K1532002 LacI BBa_B0030 araC BBa_B0015 BBa_K1532002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1532002 BBa_R0010 BBa_B0030 BBa_C0080 BBa_B0015 BBa_K1532002 | 1,10 19,218 227,241 248,1187 1196,1324 1333,1342 | BBa_K1532002 LacI BBa_B0030 araC BBa_B0015 BBa_K1532002 |