Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden, Max van 't Hof, Samantha Basalo Vazquez
Date created: 2015-09-14 11:00:00
Date modified: 2015-11-13 09:41:52
pRha + CsgA & GFP in same operon
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1583112_sequence (Version 1) |
Description
CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655.The aggregation to the nanowire ('curli') is induced by the membrane protein CsgB. CsgC, CsgE, CsgF and CsgG act as chaperones for CsgA during translation and export to the extracellular space.
This part was designed to measure intracellular expression rates of CsgA coupled to fluorescence (GFP) by cloning the biobrick I13504 into the same operon which is under control of the Rhamnose promoter.
Notes
The scar is a stop codon, but no terminator. Like this, cloning a GFP which contains an RBS we intended to directly couple CsgA expression to expression of GFP.Source
This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655| Access | Instance | Definition |
| public public | BBa_K1583100 BBa_I13504 | BBa_K1583100 BBa_I13504 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1583100 BBa_I13504 | 1,638 647,1521 | BBa_K1583100 BBa_I13504 |