Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2016-01-25 11:29:31
High constitutive promoter + CsgB & CsgC
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1583205_sequence (Version 1) |
Description
CsgB functions as the anchor on the cell wall for curli nanowires. It triggers the aggregation of CsgA and leads to the formation of amyloid nanowires. CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly. Medium expression level.Notes
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.Source
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.Non-coding scar sites were designed at random.
| Access | Instance | Definition |
| public public public public public public public public public | BBa_J23100 BBa_K1583053 BBa_K1583058 BBa_K1583054 BBa_K1583016 BBa_K1583055 BBa_B0034 BBa_K1583054 BBa_K1583001 | BBa_J23100 BBa_K1583053 BBa_K1583058 BBa_K1583054 BBa_K1583016 BBa_K1583055 BBa_B0034 BBa_K1583054 BBa_K1583001 |