Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-10 11:00:00
Date modified: 2015-09-12 12:51:52
J23119+B0034+NhaA+B0034+NhaB
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1675006_sequence (Version 1) |
Description
Construct the JBA (J23119+B0034+NhaA), B0034 and NhaB with the vector pSB1C3. Two functional genes NhaA and NhaB are used to pump in protons and lower the pH of bacteria???s microenvironment. JBA and JBB are linked with pSB1C3 through 3A Assemble successfully. Thus, it can be an alkaline-resisting part.Notes
This is a composite part including the J23119 promoter ecoded in part BBa_J23119 and the ribosome binding site B0034 ecoded in part BBa_B0034.The functional genes NhaA and NhaB are cloned from Escherichia coli str. K-12 substr. sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655
http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true
Source
The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034
The sequence of gene NhaA is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=17489&to=18655
The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true
| Access | Instance | Definition |
| public public public public public | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_B0034 BBa_K1675001 | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_B0034 BBa_K1675001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0034 BBa_K1675000 BBa_B0034 BBa_K1675001 | 1,35 44,55 64,1230 1239,1250 1257,2798 | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_B0034 BBa_K1675001 |