Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew W Mortensen
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 03:48:02
pBAD+Strong RBS+E. coli tolC signal sequence+P. aeruginosa oprM
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1683004_sequence (Version 1) |
Description
Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Pseudomonas aeruginosa PA01 tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Pseudomonas aeruginosa PA01. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.Notes
BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Pseudomonas aeruginosa PA01 TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.Source
BBa_K1406000 comes from the biobrick registry The tolC signaling sequence and Pseudomonas aeruginosa PA01 tolC sequence were synthesized from DNA sequences found in genomic databases.| Access | Instance | Definition |
| public public | BBa_K1406000 BBa_K1683005 | BBa_K1406000 BBa_K1683005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1406000 BBa_K1683005 | 1,151 152,1624 | BBa_K1406000 BBa_K1683005 |