Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olivia Smith, Adriana Collings
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:35:57
Trans-zeatin biosynthesis under control of the lac promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1702005_sequence (Version 1) |
Description
Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate into zeatin riboside 5'-phosphate. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin. Under control of the lac promoter in DH5alpha E. coli cells, expression of the trans-zeatin biosynthesis pathway is induced by lactose or its analog IPTG.Notes
The piece was synthesized with the two genes back-to-back (i.e. as an operon) and expression can be induced using lactose or IPTG.Source
This piece was synthesized based on sequences from Kamada-Nobusada, Tomoe and Sakakibara, Hitoshi. "Molecular basis for cytokinin biosynthesis." Phytochemistry 70:444-449. and from KEGG (kegg.jp).| Access | Instance | Definition |
| public public | BBa_J04500 BBa_K1702004 | BBa_J04500 BBa_K1702004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K1702004 | 1,220 229,1709 | BBa_J04500 BBa_K1702004 |