Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-09 11:00:00
Date modified: 2015-05-08 01:10:58
Degradation controller with integration site
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K174007_sequence (Version 1) |
Description
When inserted into a Bacillus subtilis integration vector, it will provide a single crossover site with arabinose controlled sspB expression. This device will be integrated onto the sacA gene on the chromosomeNotes
To test the integration, pmutin4 integration vector is selected. However after the integration pspac is left on the right side, free to drive the expression of downstream genes. To remove the effect of pspac promoter of pmutin4 vector, the device with ligation of sac integration site, araR repressible promoter and sspB CDS was amplified with PacI from one end. Hence by cutting pmutin4 with PacI, pspac promoter can be removed. For the other end, sacII was selected since it is compatible with PacI. PacI is just on the left side of Terminator0 on pmutin4, it will also be removed leaving Terminator 1 and Terminator 3 on the vector.Source
Ligated manually| Access | Instance | Definition |
| public public public | BBa_K174006 BBa_K174001 BBa_K174000 | BBa_K174006 BBa_K174001 BBa_K174000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K174006 BBa_K174001 BBa_K174000 | 1,366 375,589 596,1093 | BBa_K174006 BBa_K174001 BBa_K174000 |