BBa_K1783000

BBa_K1783000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1783000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iowis Zhu
Date created: 2015-06-02 11:00:00
Date modified: 2015-07-01 08:39:40

Miraculin Generator



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K1783000_sequence (Version 1)

    Description

    pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.

    Notes

    Parts were assembled using 3A assembly.

    Source

    All parts are from previous BioBricks. pBAD promoter is from E. coli, while Miraculin gene comes from S. dulcificum.

    Access Instance Definition
    public
    public
    		BBa_K206000
    BBa_K1033120
    		pBAD
    BBa_K1033120

    Sequence Annotation Location Component / Role(s)
    BBa_K206000
    BBa_K1033120
    		1,130
    139,855
    		pBAD
    BBa_K1033120

    igem#experience
    None
     
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1783000/1
     

    Attachments

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    | v1.6.1 (86615526)