Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Halleran, Joseph Maniaci, Caroline Golino, Taylor Jacobs, John Marken, Elli Cryan, Panya Vij, Michael LeFew
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-17 11:34:17
GalK Integrator Cassette
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1795023_sequence (Version 1) |
Description
We created a modified backbone that will allow users to quickly and easily create parts that can be integrated onto the E. coli genome. This backbone is designed as follows (where H1 refers to the first region homologous to the E. coli genome, and H2 refers to the second homologous region)In order to insert a part onto the genome, the user simply needs to put a part onto this backbone (between the prefix and suffix), and PCR up the region between (and including) H1 and H2.
Users can put their parts onto this backbone with their preferred method of assembly.
This part exhibits resistance to chloramphenicol and kanamycin. The parts integrated with this cassette will contain kanamycin resistance, and will be integrated in the galK region of the E. coli genome.
Homologous regions modeled after those in Elowitz et al 2002.
Source
iGEM Parts Registry. Homologous regions were modeled after those used in Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002). Stochastic gene expression in a single cell. Science, 297(5584), 1183-1186.| Access | Instance | Definition |
| public public public public public public | BBa_K1795400 BBa_K1795200 BBa_K1795202 BBa_K1795201 BBa_K1795024 BBa_K1795203 | BBa_K1795400 BBa_K1795200 BBa_K1795202 BBa_K1795201 BBa_K1795024 BBa_K1795203 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1795400 BBa_K1795200 BBa_K1795202 BBa_K1795201 BBa_K1795024 BBa_K1795203 | 1,2027 2028,2077 2078,2099 2100,2120 2121,3299 3300,3349 | BBa_K1795400 BBa_K1795200 BBa_K1795202 BBa_K1795201 BBa_K1795024 BBa_K1795203 |