Types | DnaRegion
|
Roles | engineered_region
Device
|
Sequences | BBa_K1797002_sequence (Version 1)
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Description
This part includes lacI and lac operator which are intended for the control of the expression of CcdB. LacI is constitutively expressed and binds to lac operator to block the expression of CcdB. In the presence of IPTG, lacI is released from operator and CcdB can be expressed. CcdB is a poisonous protein for E.coli since it affects the activity of DNA gyrase and inhibits the segregation of chromosomal DNA.
Notes
No.
Source
Escherichia coli.