BBa_K1797002

BBa_K1797002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1797002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM15_Tsinghua
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 06:59:47

IPTG-inducible CcdB



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1797002_sequence (Version 1)

Description

This part includes lacI and lac operator which are intended for the control of the expression of CcdB. LacI is constitutively expressed and binds to lac operator to block the expression of CcdB. In the presence of IPTG, lacI is released from operator and CcdB can be expressed. CcdB is a poisonous protein for E.coli since it affects the activity of DNA gyrase and inhibits the segregation of chromosomal DNA.

Notes

No.

Source

Escherichia coli.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1797002/1