BBa_K1899007

BBa_K1899007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1899007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM2016_HKUST

Date created: 2016-10-09 11:00:00

Date modified: 2016-10-18 09:47:06

phlFp-B0032-Lacl-B0032-TetR-GFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1899007_sequence (Version 1)

Description

This construct is designed to estimate the workload of E. coli and measure the real output of fluoresce by the promoter when it is ligated in tristable switch form.

Notes

We compare the RFU of this strand with the negative control( BBa_E0240, GFP generator) and PphlF-GFP, and observe the workload of E. coli.

Source

iGEM

Components

Access	Instance	Definition
public	BBa_K1899004	BBa_K1899004
public	BBa_B0032	BBa_B0032
public	BBa_C0012	lacI
public	BBa_B0032	BBa_B0032
public	BBa_C0040	tetR
public	BBa_E0240	GFP report

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1899004	1,66	BBa_K1899004
BBa_B0032	75,87	BBa_B0032
BBa_C0012	94,1221	lacI
BBa_B0032	1255,1267	BBa_B0032
BBa_C0040	1274,1958	tetR
BBa_E0240	1967,2842	GFP report

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1899007/1

Member of these Collections

• //chassis/prokaryote/ecoli
• iGEM Parts Registry