BBa_K1913030

BBa_K1913030 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1913030
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianhe Wang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 03:58:43

Synthetic plac-FixK2 hybrid promoter+RBS with mRFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1913030_sequence (Version 1)

Description

Synthetic plac-FixK2 hybrid promoter composite part includes mRFP reporter (BBa_E1010). After co-transformation with light sensor BBa_K1913033, mRFP fluorescence could be detected after 12h culture.

Notes

In order to test synthetic plac-FixK2 hybrid promoter,we put reporter mRFP under this promoter and co-transformed with light sensor BBa_K1913034. And mRFP fluorescence value was tested so that the intensity of transcription of this promoter could be quantified.

Source

Genomic sequence of Bradyrhizobium japonicum, Genomic sequence of E.coli, iGEM part registry.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1913030/1