BBa_K1932005

BBa_K1932005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1932005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jin Liu

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-16 02:00:05

This device is made up of BBa_K1932000
BBa_K1932001
BBa_K1932004

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1932005_sequence (Version 1)

Description

The device is designed to replicate stably and express robustly of the TAT-apoptin. Among the subparts, BBa_K1932000 is added to up-regulate the expression of exogenous protein in Bifidobacterium with the presence of a strong promoter. BBa_K1932001 is constructed into the device to increase the stability of the device in Bifidobacterium. BBa_K1932004 encodes TAT-apoptin, which acts as the effector protein to kill the cancer cells.

Notes

（1）The restriction enzyme cutting sites were identified.
（2）The order of the parts was confirmed.

Source

Gene pool

Components

Access	Instance	Definition
public	BBa_K1932000	BBa_K1932000
public	BBa_K1932001	BBa_K1932001
public	BBa_K1932004	BBa_K1932004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1932000	1,116	BBa_K1932000
BBa_K1932001	125,1866	BBa_K1932001
BBa_K1932004	1873,2238	BBa_K1932004

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1932005/1

Member of these Collections

• iGEM Parts Registry