Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jin Liu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-16 02:01:37
This device is constructed to express TAT-apoptin fused with sec2.
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1932006_sequence (Version 1) |
Description
This device was cloned to express the fusion protein, TAT-apoptin and the sec2 peptide coding sequence was included to increase the secretion of the protein. Among the subparts, BBa_1932000 is a strong promoter to regulate the expression of exogenous protein in Bifidobacterium. BBa_1932001 is included to enhance the stability of the device in Bifidobacterium. BBa_193202 is the sequence of the signal peptide, sec2, that can direct the export of the protein from Bididobacterium. BBa_1932004 encodes the TAT-apoptin fusion protein, which can induce the apoptosis specifically in the cancer cells.Notes
（1）The restriction enzyme cutting site were identified.（2）The order of the parts are confirm
Source
gene pool| Access | Instance | Definition |
| public public public public | BBa_K1932000 BBa_K1932001 BBa_K1932002 BBa_K1932004 | BBa_K1932000 BBa_K1932001 BBa_K1932002 BBa_K1932004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1932000 BBa_K1932001 BBa_K1932002 BBa_K1932004 | 1,116 125,1866 1875,2008 2015,2380 | BBa_K1932000 BBa_K1932001 BBa_K1932002 BBa_K1932004 |