Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jin Liu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-16 02:02:25
This device is constructed for the expression of TAT-apoptin fused with tmp1.
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1932007_sequence (Version 1) |
Description
This device is constructed to express the fusion protein, TAT-apoptin and the region of tmp1 is included to increase the secretion quantity of the protein. Among the subparts, BBa_1932000 is added as a promoter to regulate the expression of exogenous protein in Bifidobacterium with. BBa_1932001 is included into the device to increase the stability of the device in Bifidobacterium. BBa_193203 encodes the signal peptide, tmp1, which can direct the export of the protein from Bididobacterium. BBa_1932004 encodes TAT-apoptin, which acts as the effector protein to kill the cancer cells.Notes
（1）The restriction enzyme cutting sites were identified.（2）The order of the parts was confirm
Source
gene pool| Access | Instance | Definition |
| public public public public | BBa_K1932000 BBa_K1932001 BBa_K1932003 BBa_K1932004 | BBa_K1932000 BBa_K1932001 BBa_K1932003 BBa_K1932004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1932000 BBa_K1932001 BBa_K1932003 BBa_K1932004 | 1,116 125,1866 1875,2071 2078,2443 | BBa_K1932000 BBa_K1932001 BBa_K1932003 BBa_K1932004 |