Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomoki Uchino
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-17 04:57:35
constitutive expression of anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1933200_sequence (Version 1) |
Description
anti-Norovirus GII.4 scFv fused to INPNC with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a single chained variable fragment(scFv) of a norovirus GII.4 strain fused to surface expression anchoring domain(INPNC), connected by a poly-histidine tag to be easily identified by Western blotting. For more information, please visit our Wiki page.Notes
Silent mutation was done to remove PstI site.Source
Promoter and RBSThe INPNC surface expression component derives from Pseudomonas Syringae. We used parts BBa_K811005 from iGEM12_Penn. scFv is made from fusing variable region (VH and VL chain) of a human anti-norovirus antibody with a linker peptide. We have been given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
| Access | Instance | Definition |
| public public public | BBa_K1933004 BBa_K1933011 BBa_K1933002 | BBa_K1933004 BBa_K1933011 scFv |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1933004 BBa_K1933011 BBa_K1933002 | 1,60 61,1035 1036,1815 | BBa_K1933004 BBa_K1933011 scFv |