Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tomoki Uchino
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-10 12:42:57
constitutive expression of anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1933201_sequence (Version 1) |
Description
anti-Norovirus GII.4 scFv fused to BclA with 6xHis tag is one of a series of surface expressing fusion proteins that make up biodevice that aims to be therapeutic solution against norovirus infections. This protein in particular is a single chained variable fragment(scFv) of a norovirus GII.4 strain fused to surface expression anchoring domain(BclA), connected by a 6xHis tag to be easily identified by Western blotting. For more information, please visit our Wiki page.Notes
Silent mutation was done to remove PstI site.Source
Promoter and RBS:The BclA surface expression component derives from Bacillus anthracis(34F2), it is a hair-like protein that covers B. anthracis??? spores. We outsourced chemical synthesis of this part for construction. scFv is made from fusing variable region (VH and VL chain) of a human anti-norovirus antibody with a linker peptide. We have been given the scFv-encoding plasmid (NoV GII.4 strain-specific antibody 12A2 (vector: pSCCA5-E8d)) from Dr. Kyoko Moriguchi of the Fujita Health University Department of Virology Parasitology.
| Access | Instance | Definition |
| public public public | BBa_K1933004 BBa_K1933000 BBa_K1933002 | BBa_K1933004 BclA-His scFv |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1933004 BBa_K1933000 BBa_K1933002 | 1,60 61,144 145,924 | BBa_K1933004 BclA-His scFv |