BBa_K1940001

BBa_K1940001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1940001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yujia Liu

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-14 06:36:27

microRNA-34c detector 1.0

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_K1940001_sequence (Version 1)

Description

We designed this part to detect the abundance of microRNA-34c. Anti-microRNA-34c ( BBa_K1940000) was insert after a constitutive promoter (BBa_J23106) which express GFP. As anti- microRNA-34c is sensitive to the expression levels of miR-34c, we would have a direct impact on GFP???s expression. Determining GFP???s fluorescence intensity differences will justify the content amount of microRNA-34c.

Notes

We had tested that anti-microRNA-34c be put in this position was most effective than be put after RBS or GFP.

Source

composited

Components

Access	Instance	Definition
public	BBa_J23106	BBa_J23106
public	BBa_K1940000	BBa_K1940000
public	BBa_B0034	BBa_B0034
public	BBa_E0040	GFP
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23106	1,35	BBa_J23106
BBa_K1940000	44,194	BBa_K1940000
BBa_B0034	203,214	BBa_B0034
BBa_E0040	221,940	GFP
BBa_B0015	949,1077	BBa_B0015

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1940001/1

Member of these Collections

• iGEM Parts Registry