Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Justine Hsu
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 08:21:44
Leishmania 5 prime UTR-HYG-OVA-3 prime UTR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1955006_sequence (Version 1) |
Description
OVA has been commonly used as the antigen for testing the efficiency of antibody response and T cell activation in previous immunology experiments, thus is a easy to use antigen. We add the OVA coding region in between the Leish-5'UTR-HYG and Leish-3'UTR for leishmania to expression our target protein, OVA.Notes
The detailed design of the sequence is documented in each part.Source
Composite of the Leishmania 5 prime UTR-HYG, ovalbumin and 3 prime UTR. The Leish-5'UTR and 3'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The Hygromycin resistant gene and ovalbumin sequences are acquired from GenBank.| Access | Instance | Definition |
| public public public | BBa_K1955003 BBa_K1955004 BBa_K1955002 | BBa_K1955003 BBa_K1955004 BBa_K1955002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1955003 BBa_K1955004 BBa_K1955002 | 1,1446 1455,3567 3576,4349 | BBa_K1955003 BBa_K1955004 BBa_K1955002 |