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BBa_K195617

BBa_K195617 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K195617

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yun-Hsiang Chang

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:17

pTet with activator gene CII and pRE with GFP generator

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K195617_sequence (Version 1)

Description

This part is composed of pTetR with inducer gene CII, and it would induce pRE to produce green fluorescent protein. The coding sequence CII could induce BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).The pRE would be induced by CII produced by the downstream gene of the regulatory promoter pTet and produce green fluorescent protein.

Notes

none

Source

biobrick

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	p(tetR) BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0010 BBa_B0012 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,54 63,74 81,374 383,462 471,511 520,567 576,587 594,1313 1322,1401 1410,1450	p(tetR) BBa_B0034 CII BBa_B0010 BBa_B0012 pRE BBa_B0034 GFP BBa_B0010 BBa_B0012

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K195617/1