Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laetitia Warny
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:17
Final GluColi device.
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K196014_sequence (Version 1) |
Description
This is the part we actually created to test the glue production by E. coli.HfsG and HfsH come from Caulobacter crescentu. Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase.
RFP has been added as a reporter.
Notes
As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by GeneArt [2]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.Source
HfsG and HfsH sequences come from Caulobacter crescentus.| Access | Instance | Definition |
| public public | BBa_K196007 BBa_K196005 | BBa_K196007 BBa_K196005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K196007 BBa_K196005 | 1,787 794,2646 | BBa_K196007 BBa_K196005 |