| Types | DnaRegion
|
| Roles | engineered_region
Generator
|
| Sequences | BBa_K1961004_sequence (Version 1)
|
Description
The Pb2+ biosensor involves (1) generation of PbrR promoter with a binding site for lead ion, thus forming Pb2+-PbrR dimer and (2) GFP expression when Pb2+-PbrR dimer binds onto pbr promoter. Because the PbrR and Pb2+PbrR dimer compete to bind onto pbr promoters and result in poor GFP performance, we designed an autoregulated PbrR generator (BBa_K1961004) to control the amount of PbrR. This composite part consists of the TetR and PbrR generators, both controlled by PTet, which has TetR binding sites. TetRs bind onto PTet and repress the gene expression of TetR, PbrR and GFP. We used tetracycline to induce the gene expression of PbrR and GFP.
Notes
No
Source
We use parts in the 2016 iGEM plate
