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BBa_K1961006

BBa_K1961006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1961006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yi-Ting Lin

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-11 08:44:20

Lead ion detector

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K1961006_sequence (Version 1)

Description

This part is designed to detect lead ion following the concept of B. It consists of two parts:
(1) PbrR generation:this part produces PbrR which can form a dimer of Pb2+/PbrR in the presence of Pb2+.
(2) RFP expression: this part is controlled by a promoter(BBa_I721001) which has a binding site of dimer of Pb2+/PbrR. RFP expression can be observed when the dimer binds onto the promoter.

Notes

We followed the design used by the HSNU-Taipei.

Source

NCBI

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0010 BBa_B0012 BBa_I721001 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0010 BBa_B0012 BBa_I721001 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0010 BBa_B0012 BBa_I721001 BBa_B0034 BBa_E1010 BBa_B0010 BBa_B0012	1,35 44,56 63,461 470,549 558,598 607,700 709,720 727,1432 1441,1520 1529,1569	BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0010 BBa_B0012 BBa_I721001 BBa_B0034 mRFP1 BBa_B0010 BBa_B0012