BBa_K1972014

BBa_K1972014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1972014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 07:38:06

dszBBAC with tac promoter and dszD with lac promotor induced by IPTG



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1972014_sequence (Version 1)

Description

We constructed dszBCAD genes with T7 promoter and IPTG controled system and choose E.coli as our host cell.T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and dszBCAD genes with T7 promoter will be greatly expressed by the host cell. The four enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.


Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)

Notes

Not yet.

Source

Each single biodesulfurization enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
The T7 RNA polymerase controled system is PCRed from E.coli Bl21.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1972014/1