Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriel Ruiz Romero
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 09:28:12
miniTn7BB-Gm-nahR-Psal-nasF-lapG
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1973009_sequence (Version 1) |
Description
nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter in the presence of salicylate. lapG (see BBa_K1973000) is expressed from the Psal promoter (see BBa_J61051) in the presence of this molecule. nasF (see BBa_K1973001) is an attenuator of transcription elongation (terminator) from Klebsiella pneumoniae that decreases the basal expression from the Psal promoter. The miniTn7 tool allows the stable integration of this module in the chromosome of a wide range of bacteria (see http://parts.igem.org/Genome_Integration).Notes
Designs are described in basic parts pagesSource
Basic parts were cloned into the miniTn7BB-Gm Backbone| Access | Instance | Definition |
| public public public | BBa_K510000 BBa_K1973005 BBa_K1973000 | BBa_K510000 BBa_K1973005 BBa_K1973000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K510000 BBa_K1973005 BBa_K1973000 | 1,4388 4397,5547 5556,6218 | BBa_K510000 BBa_K1973005 BBa_K1973000 |