BBa_K1991008

BBa_K1991008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1991008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chen, Pei-En
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:17:39

Pcons-RBS-LO-AOX1-His



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1991008_sequence (Version 1)

Description

A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.

Notes

No

Source

E. coli (Lpp-OmpA) & Pichia pastoris (AOX)

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1991008/1