Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Celia Chenebault, Camille Soucies, Benjamin Piot
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-20 12:05:53
Biosensor device for BTEX detection
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K2023001_sequence (Version 1) |
Description
The Pr promoter is a constitutive promoter driving the transcription of the XylR gene, coding for the XylR protein. The XylR protein, a transcriptional regulatory protein for the Pu promoter, is activated by aromatic hydrocarbons that carry a methyl group (like toluene and xylene). In our biosensor the Pu promoter allows the transcription of the bioluminescent reporter gene GLuc, coding for the Gaussia luciferase. When this enzyme reacts with its coelenterazine substrate it emits luminescence.Notes
Site directed mutagenesis to overcome the mutation in the start codon of XylRSource
IDT synthesis in 3 parts that were then assembled| Access | Instance | Definition |
| public public | BBa_K2023002 BBa_K2023003 | BBa_K2023002 BBa_K2023003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2023002 BBa_K2023003 | 1,2243 2252,3261 | BBa_K2023002 BBa_K2023003 |