| Types | DnaRegion
|
| Roles | plasmid
Plasmid
|
| Sequences | BBa_K2033011_sequence (Version 1)
|
Description
This is a modular sender vector designed to interchange the AHL inducer sequence and serve as a standard backbone for most AHL systems. The part begins with a pTet promoter, followed by 2 ribosome binding sites, followed by mCherry and a terminator. The AHL will insert between the two RBSs. Ampicillin Resistance (AmpR) is also on the plasmid.
Notes
A suitable RBS was difficult to find. The binding affinity either resulted in overflow readings or minimal readings. The RBS B0034 was found to produce appropriate reading levels.
Source
This part was designed with the help of Dr. Karmella Haynes, Ryan Muller, Cassandra Barrett, and Rene Davis.
