BBa_K206011

BBa_K206011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K206011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Amelia Hardjasa

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:24

Jammer proof of concept (J23101)

Details

• Types: DnaRegion
• Roles: Device; engineered_region
• Sequences: BBa_K206011_sequence (Version 1)

Description

This is a proof of concept construct for the jammer system, showing gene knockdown via reverse promoter transcription.

Notes

GFP is constitutively transcribed and expressed from the constitutive promoter J23101(relative strength 1791). Upon the addition of arabinose, pBAD induces transcription of a reverse transcript. Binding of the reverse and forward transcript result in knockdown (i.e. decreased expression) of reporter gene transcription, either through signaling for degradation or simple hybridization preventing translation of the mRNA.

Source

Assembled from Registry BioBricks.

Components

Access	Instance	Definition
public	BBa_B0012	BBa_B0012
public	BBa_B0011	BBa_B0011
public	BBa_J23101	BBa_J23101
public	BBa_B0034	BBa_B0034
public	BBa_K145015	GFP
public	BBa_J44002	BBa_J44002
public	BBa_B0012	BBa_B0012
public	BBa_B0011	BBa_B0011

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0012	1,41	BBa_B0012
BBa_B0011	50,95	BBa_B0011
BBa_J23101	104,138	BBa_J23101
BBa_B0034	147,158	BBa_B0034
BBa_K145015	165,923	GFP
BBa_J44002	932,1061	BBa_J44002
BBa_B0012	1070,1110	BBa_B0012
BBa_B0011	1119,1164	BBa_B0011

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K206011/1

Member of these Collections

• iGEM Parts Registry