Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-31 11:00:00
Date modified: 2016-10-14 09:02:52
plLac0-1+RiboJ+cI-GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2066015_sequence (Version 1) |
Description
This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship of IPTG-cIGFP.Notes
We made this part for our Ribozyme Characterization project to see the effect of adding a RiboJ (ribozyme insulator) sequence in the 5' UTR on the input-ouput relationship (transfer function).Source
The promoter, RBS, RiboJ, and cI-GFP sequences were derived and modified from Lou et. al 2012 "Ribozyme-based insulator parts buffer synthetic circuits from genetic context". The UNS sequences are from Torella et al. 2013.| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K2066508 BBa_K2066506 BBa_K2066510 BBa_K2066511 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K2066508 BBa_K2066506 BBa_K2066510 BBa_K2066511 BBa_B0015 BBa_K2066019 |