Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Date created: 2016-08-31 11:00:00
Date modified: 2016-10-28 08:36:39
pTac+RiboJ+cIGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2066025_sequence (Version 1) |
Description
This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. This part will be used in concert with WM16_024 (pTac Ribozyme Chracterization Part ???GFP Only) to determine the influence of the RiboJ insulator sequence on gene expression.Notes
This part follows Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).Source
The RBS, RiboJ, and reporter sequences are from Lou et. al. 2012. The UNS sequences are from Torella et. al 2013.| Access | Instance | Definition |
| public public public public public public public | BBa_K2066018 BBa_K864400 BBa_K2066506 BBa_K2066515 BBa_K2066511 BBa_B0015 BBa_K2066019 | BBa_K2066018 BBa_K864400 BBa_K2066506 BBa_K2066515 BBa_K2066511 BBa_B0015 BBa_K2066019 |